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KMID : 1161420130160050410
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Journal of Medicinal Food
2013 Volume.16 No. 5 p.410 ~ p.420
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Chloroform Extract of Alfalfa (Medicago sativa) Inhibits Lipopolysaccharide-Induced Inflammation by Downregulating ERK/NF-κB Signaling and Cytokine Production
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Choi Ki-Choon
Hwang Jung-Min
Bang Sung-Jun
Kim Beom-Tae
Kim Dong-Hern
Chae Min-Seon
Lee Seung-Ah
Choi Gi-Jun
Kim Da-Hye
Lee Jeong-Chae
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Abstract
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Alfalfa (Medicago sativa L.) is commonly used as a traditional medicine and functional food. This study investigated the anti-inflammatory potential of alfalfa and the mechanisms involved. The chloroform extract of alfalfa aerial parts inhibited lipopolysaccharide (LPS)-stimulated immune responses more than ether, butanol, or water soluble extracts. Treatment with 1 μg/mL LPS increased nitrite concentrations to 44.3 μM in RAW267.4 macrophages, but it was reduced to 10.6 μM by adding 100 μg/mL chloroform extract. LPS treatment also increased the concentrations of tumor necrosis factor-α, interleukin (IL)-6, and IL-1β to 41.3, 11.6, and 0.78?ng/mL in culture supernatants of the cells, but these cytokine levels decreased to 12.5, 3.1, and 0.19?ng/mL, respectively, by pretreating with 100 μg/mL of the extract. ICR mice injected with LPS (30?mg/kg body weight) alone showed a 0% survival rate after 48?h of the injection, but 48-h survival of the mice increased to 60% after oral administration of the extract. Subfractions of the chloroform extract markedly suppressed LPS-mediated activation of the extracellular signal-regulated kinase and nuclear factor kappa-B. Cinnamic acid derivatives and fatty acids were found to be active constituents of the extract. This research demonstrated that alfalfa aerial parts exert anti-inflammatory activity and may be useful as a functional food for the prevention of inflammatory disorders.
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KEYWORD
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anti-inflammation, bioactive compounds, health functional food, nitric oxide, nuclear factor kappa-B
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